What is the correct sequence of events in lagging strand replication?

What is the correct sequence of events in lagging strand replication?

• A. 1 -> 6 -> 2 -> 4 -> 3
• B. 1 -> 6 -> 8 -> 4 -> 3
• C. 1 -> 5 -> 2 -> 7 -> 3
• D. 1 -> 5 -> 8 -> 7 -> 3

Answer: (C)

In the process of DNA replication, the lagging strand is synthesized in a discontinuous fashion, creating fragments known as Okazaki fragments. The correct sequence of events in lagging strand replication typically involves several key steps that correspond directly to the mechanisms of DNA synthesis. Beginning with the identification of the template strand, the initial step includes unwinding of the DNA helix and the formation of a replication fork. Following this, RNA primers are synthesized by primase to provide a free 3' hydroxyl (-OH) group, which is crucial for DNA polymerase to start synthesis. After the primer is laid down, DNA polymerase extends the newly forming strand by adding nucleotides complementary to the template strand. This process occurs in a series of steps such that the DNA polymerase works away from the replication fork. Consequently, once an Okazaki fragment is completed, the RNA primer is removed and replaced with DNA. The final steps involve the sealing of the Okazaki fragments by DNA ligase, which joins any nicks in the sugar-phosphate backbone. In the stated sequence, starting from step one and following through to five, signifies that the correct order of events—starting from primer synthesis and leading to the completion of Okazaki fragments

In the process of DNA replication, the lagging strand is synthesized in a discontinuous fashion, creating fragments known as Okazaki fragments. The correct sequence of events in lagging strand replication typically involves several key steps that correspond directly to the mechanisms of DNA synthesis.

Beginning with the identification of the template strand, the initial step includes unwinding of the DNA helix and the formation of a replication fork. Following this, RNA primers are synthesized by primase to provide a free 3' hydroxyl (-OH) group, which is crucial for DNA polymerase to start synthesis. After the primer is laid down, DNA polymerase extends the newly forming strand by adding nucleotides complementary to the template strand.

This process occurs in a series of steps such that the DNA polymerase works away from the replication fork. Consequently, once an Okazaki fragment is completed, the RNA primer is removed and replaced with DNA. The final steps involve the sealing of the Okazaki fragments by DNA ligase, which joins any nicks in the sugar-phosphate backbone.

In the stated sequence, starting from step one and following through to five, signifies that the correct order of events—starting from primer synthesis and leading to the completion of Okazaki fragments